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English: a Single-cell assay for transposase-accessible chromatin (scATAC-seq) methods. Key steps of the ATAC-seq protocol are shown in the middle column. The left panel summarizes the scATAC-seq protocol based on cellular indexing established by Cusanovich et al. [4]. The right panel illustrates the microfluidic-based protocol introduced by Buenrostro et al. [3]. FACS fluorescence-activated cell sorting. b ATAC-seq from single cells is sparse and near binary. The properties of chromatin accessibility data from pooled cells (DNase hypersensitive sites (DHS) data from GM12878 cells on the top in dark blue) and a cartoon representation of how reads from scATAC-seq data might be distributed throughout the same genomic region
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Source https://genomebiology.biomedcentral.com/articles/10.1186/s13059-015-0737-7
Author Sebastian Pott and Jason D. Lieb
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