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English: Figure 6. Stepwise assembly of a DEL library using YoctoReactor technology. A 3-way reactor is shown here. (a) Position 1 (P1) and P2 BB are brought into close proximity and undergo a chemical reaction in the presence of a helper oligonucleotide in P3. (b) The structure is purified by polyacrylamide gel electrophoresis (PAGE), the P1 and P2 DNA is ligated and the P2 linker is cleaved. (c) P3 BB is annealed to the P1-P2 ligation product from step b, and a chemical reaction between P2 and P3 BBs takes place. (d) The reaction product is purified by PAGE, the DNA is ligated and P3 linker is cleaved yielding a compound (OOO) covalently attached to the folded yR. (e) The yR is dismantled by primer extension yielding a double-stranded disply product exposing the reaction product for selection and molecular evolution.
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Author Viptrh

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21 May 2010

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Date/TimeThumbnailDimensionsUserComment
current08:38, 25 May 2010Thumbnail for version as of 08:38, 25 May 2010288 × 720 (22 KB)Viptrhimproved small details of image
08:36, 25 May 2010Thumbnail for version as of 08:36, 25 May 2010288 × 720 (22 KB)Viptrhimproved small details of image
08:33, 25 May 2010Thumbnail for version as of 08:33, 25 May 2010288 × 720 (22 KB)ViptrhImproved small details of image
08:03, 25 May 2010Thumbnail for version as of 08:03, 25 May 2010288 × 720 (22 KB)ViptrhImproved small details of image.
08:00, 25 May 2010Thumbnail for version as of 08:00, 25 May 2010288 × 720 (22 KB)ViptrhImproved small details of image.
07:56, 25 May 2010Thumbnail for version as of 07:56, 25 May 2010288 × 720 (22 KB)Viptrhpowerpoint image improved. Small details.
11:45, 21 May 2010Thumbnail for version as of 11:45, 21 May 2010288 × 720 (21 KB)Viptrh{{Information |Description={{en|1=Figure 6. Stepwise assembly of a DEL library using YoctoReactor technology. A 3-way reactor is shown here. (a) Position 1 (P1) and P2 BB are brought into close proximity and undergo a chemical reaction in the presence of

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